Publications [#323019] of Sally A. Kornbluth

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Papers Published

  1. Kornbluth, S; Yang, J; Powers, M, Analysis of the cell cycle using Xenopus egg extracts., Current protocols in cell biology, vol. Chapter 11 (January, 2006), pp. Unit-11.11 [doi] .
    (last updated on 2024/11/03)

    Abstract:
    In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.