Publications [#323049] of Sally A. Kornbluth
search PubMed.Papers Published
- Kaye, FJ; Modi, S; Ivanovska, I; Koonin, EV; Thress, K; Kubo, A; Kornbluth, S; Rose, MD, A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch.,
FEBS letters, vol. 467 no. 2-3
(February, 2000),
pp. 348-355 [doi] .
(last updated on 2024/11/03)Abstract:
We have isolated two human ubiquitin-like (UbL) proteins that bind to a short peptide within the ATPase domain of the Hsp70-like Stch protein. Chap1 is a duplicated homologue of the yeast Dsk2 gene that is required for transit through the G2/M phase of the cell cycle and expression of the human full-length cDNA restored viability and suppressed the G2/M arrest phenotype of dsk2Delta rad23Delta Saccharomyces cerevisiae mutants. Chap2 is a homologue for Xenopus scythe which is an essential component of reaper-induced apoptosis in egg extracts. While the N-terminal UbL domains were not essential for Stch binding, Chap1/Dsk2 contains a Sti1-like repeat sequence that is required for binding to Stch and is also conserved in the Hsp70 binding proteins, Hip and p60/Sti1/Hop. These findings extend the association between Hsp70 members and genes encoding UbL sequences and suggest a broader role for the Hsp70-like ATPase family in regulating cell cycle and cell death events.