Publications [#383005] of Lucia C. Strader
search PubMed.Papers Published
- Allen, JR; Flanagan, M; Pathak, S; Emenecker, SK; Emenecker, RJ; Strader, LC, An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.,
BMC research notes, vol. 18 no. 1
(April, 2025),
pp. 149, Springer Science and Business Media LLC [doi] .
(last updated on 2026/01/19)Abstract:
Objective
Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection.Results
Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community.